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MICROFLUIDIC ARRAY DEVICE AND SYSTEM FOR SIMULTANEOUS DETECTION OF MULTIPLE ANALYTES

机译:用于同时检测多种分析物的微流控阵列装置和系统

摘要

(A1+A3, B1-B3, C1-C3) Disclosed herein are microfluidic devices having an array of microfluidic valves and other components to meet the requirement of an antibody array for analyte detection. The microfluidic valves disclosed herein enable simultaneous detection of multiple analytes in a sample. One embodiment exemplified herein pertains to a microarray that is in the format of a sandwich assay, each of which comprises a capture antibody, analyte, and secondary detection antibody conjugated with a fluorescent dye or an enzyme or another moiety to facilitate detection. Methods of using microfluidic valves in an array for simultaneously detecting multiple analytes is also disclosed.
机译:(A1 + A3,B1-B3,C1-C3)本文公开了具有微流体阀阵列和其他组件的微流体装置,以满足用于分析物检测的抗体阵列的要求。本文公开的微流体阀使得能够同时检测样品中的多种分析物。本文示例的一个实施方案涉及呈夹心测定法形式的微阵列,其每个包含捕获抗体,分析物和与荧光染料或酶或另一部分缀合以促进检测的第二检测抗体。还公开了使用阵列中的微流阀同时检测多种分析物的方法。

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