Modification of Periodate Oxidation Method to Produce HRP-IgG Conjugate and Test its Stability Overtime

Nataliia Pavliuchenko; Victoria Hazarnian; Marcel Bassil

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Modification of Periodate Oxidation Method to Produce HRP-IgG Conjugate and Test its Stability Overtime

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摘要

Goat anti-Rabbit Horseradish Peroxidase (HRP) secondary conjugate was produced using a modified periodate oxidation method. The obtained conjugate was tested in the quality control techniques of therapeutic proteins. To determine the working dilution, titration of the prepared conjugate was performed in Indirect Enzyme-Linked Immunosorbent Assay (ELISA) and found to be 1:5000. This dilution was further tested in Western Blot analysis. The secondary conjugate was kept at 4°C for one month and its stability was verified by Western Blot and Indirect ELISA techniques.

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《美国分子生物学期刊（英文）》 |2019年第2期|52-63|共12页
作者
Nataliia Pavliuchenko; Victoria Hazarnian; Marcel Bassil;
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作者单位

Biotechnology Department;

Benta Pharma Industries;

Dbayeh;

Lebanon;

Faculty of Pharmacy;

Lebanese University;

Rafic Hariri University Campus;

Hadath;

Lebanon;

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原文格式 PDF
正文语种 chi
中图分类肿瘤学;
关键词
Antibody; Conjugation; Horseradish; Peroxidase; (HRP); Indirect; ELISA; Periodate-Mediated; Glycoprotein; Oxidation;

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Modification of Periodate Oxidation Method to Produce HRP-IgG Conjugate and Test its Stability Overtime

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