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首页> 外文学位 >SIMULTANEOUS DETERMINATION OF PYRIDOXINE, RIBOFLAVIN, AND THIAMINE IN FORTIFIED CEREAL PRODUCTS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC).
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SIMULTANEOUS DETERMINATION OF PYRIDOXINE, RIBOFLAVIN, AND THIAMINE IN FORTIFIED CEREAL PRODUCTS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC).

机译：高效液相色谱法（HPLC）同时测定强化谷类产品中的吡OX醇，核黄素和硫胺素。

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摘要

High performance liquid chromatography is used to simultaneously determine pyridoxine, riboflavin, and thiamine in fortified cereal products. The vitamins are extracted into dilute acid, followed by enzymatic treatment of the extract with an amylase preparation. No clean-up or pre-concentration of sample extracts is required. The vitamins are separated from other sample components on a reverse phase (ODS) column, using an ion-pairing mechanism. An acidified methanol/water mobile phase containing hexanesulfonate ion as the ion-pairing agent is used to achieve the separation. Pyridoxine and riboflavin are quantitated by fluorescence detection using a 288 nm excitation wavelength. The fluorescent thiochrome derivative of thiamine is formed by addition of an alkaline ferricyanide solution in a post-column reaction system. Thiamine is quantitated as thiochrome by use of a second fluorescence detector. A column switching technique, utilizing a short 50 mm column in conjunction with a six-port high pressure switching valve and the analytical column, is used to minimize the required chromatographic time. Use of the column switching technique allows highly retained components to be eluted from the short column directly to waste, thereby limiting the required chromatographic time to less than 25 min.;The HPLC technique provides better selectivity for riboflavin than do the wet chemical techniques, as the wet chemical procedures rely on chemical reactions to eliminate interferences, and these reactions may not be totally specific for riboflavin. HPLC determination of thiamine provides separation of thiamine from other reducing substances in an extract, such as reducing sugars produced during extraction of sucrose sweetened products, which would otherwise interfere with thiochrome formation. The improved specificity of the HPLC technique provides greater reliability than can be obtained with the wet chemical procedures. Significant time savings are also obtainable over individual, manual, wet chemical assays.;Simultaneous HPLC determination of pyridoxine, riboflavin, and thiamine provides a practical technique for routine, day-to-day usage in quality control applications.;Detection limits for the analytical procedure are 2 (mu)g pyridoxine/g sample, 1 (mu)g riboflavin/g, and 1 (mu)g thiamine/g. Relative standard deviations of the analytical procedure are 1.66%, 1.51%, and 2.06% for pyridoxine, riboflavin, and thiamine, respectively. Comparison of HPLC analytical results with wet chemical values obtained on a set of fortified cereal product samples resulted in correlations of 0.996, 0.998, and 0.996 for pyridoxine, riboflavin, and thiamine, respectively.
机译：高效液相色谱法可同时测定强化谷物产品中的吡ido醇，核黄素和硫胺素。将维生素提取到稀酸中，然后用淀粉酶制剂对提取物进行酶处理。无需对样品提取物进行净化或预浓缩。使用离子对机制，将维生素与其他样品组分在反相（ODS）色谱柱上分离。使用含有己烷磺酸根离子作为离子对剂的酸化甲醇/水流动相进行分离。吡rid醇和核黄素通过使用288 nm激发波长的荧光检测进行定量。通过在柱后反应系统中添加碱性铁氰化物溶液来形成硫胺素的荧光硫色素衍生物。通过使用第二个荧光检测器将硫胺素定量化为硫代色素。色谱柱切换技术结合了50毫米短色谱柱，六通高压切换阀和分析柱，可最大程度地缩短所需的色谱分析时间。使用色谱柱切换技术可使高度保留的组分直接从短色谱柱上洗脱下来，从而浪费，从而将所需的色谱时间限制在25分钟以内。HPLC技术对核黄素的选择性优于湿化学技术，因为湿化学程序依靠化学反应消除干扰，这些反应可能对核黄素不是完全特异的。 HPLC测定硫胺素可将硫胺素与提取物中的其他还原性物质分离，例如在蔗糖甜味产品提取过程中产生的还原糖，否则会干扰硫色素的形成。 HPLC技术提高的特异性提供了比湿化学方法更高的可靠性。通过单独的，手动的，湿式化学分析也可节省大量时间。同时HPLC测定吡ido醇，核黄素和硫胺素为质量控制应用中的日常常规使用提供了一种实用技术。程序为2微克吡ido醇/克样品，1微克核黄素/克和1微克硫胺素/克。吡ido醇，核黄素和硫胺素的分析方法的相对标准偏差分别为1.66％，1.51％和2.06％。 HPLC分析结果与在一组强化谷物产品样品上获得的湿化学值的比较导致吡ido醇，核黄素和硫胺素的相关性分别为0.996、0.998和0.996。

著录项

  • 作者

    WEHLING, RANDY LEE.;

  • 作者单位

    Kansas State University.;

  • 授予单位 Kansas State University.;
  • 学科 Agriculture Food Science and Technology.;Chemistry Analytical.
  • 学位 Ph.D.
  • 年度 1983
  • 页码 111 p.
  • 总页数 111
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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