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Characterization of dura mater and cranial suture cell phenotype.

机译：硬脑膜和颅缝细胞表型的表征。

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摘要

Skull bone formation occurs by either a direct intramembraneous or an indirect endochondral process. Both processes involve mesenchymal cells differentiation, synthesis and mineralization of extracellular matrix. Cranial vault forms through intramembraneous ossification of the surface mesenchyme surrounding the developing brain by direct transformation of mesenchymal cells into osteoblasts. Sutures are fibrous joints where two or more cranial and/or facial bones meet and they are found only in the skull. They enable longitudinal growth of the craniofacial bones and without them skull can grow thicker but not longer. Dura mater plays an important role in morphogenesis and phenotypic maintenance of cranial sutures and calvarial bone formation. Any alteration (genetic or environmental) in tissue interactions among the brain, dura mater, suture mesenchyme, and calvarial bones will affect skull bone formation and/or premature suture obliteration (craniosynostosis). The complexity of dura mater and cranial suture roles on brain expansion, calvarial bone formation and bone healing leads to the assumption of the presence of mesenchymal stem cells within these tissues.; Neonatal dura mater and cranial suture cells were isolated, cultured and differentiated into several restricted lineages. These cells were able to undergo osteogenic, chondrogenic, and adipogenic differentiation under specific stimulation. Adult dura mater cells failed to differentiate into chondrocytes and adipocytes. Positive staining with Stro-1 antibody and flow eytometric analysis further support the existence of cells with multiple differentiation potentials within neonatal dura mater and cranial suture tissues. However, adult dura mater failed to express Stro-I antigen.; In conclusion, a subpopulation of cells with characteristics of mesenchymal stem cells exists within neonatal dura mater and cranial suture tissues, and this population decreases significantly or is absent in adult dura mater tissue.
机译：颅骨的形成是通过直接的膜内或间接的软骨内过程发生的。这两个过程都涉及间充质细胞的分化，细胞外基质的合成和矿化。颅顶穹through是通过将间充质细胞直接转化为成骨细胞而围绕发育中的大脑周围的表面间充质进行膜内骨化而形成的。缝合线是两个或多个颅骨和/或面骨相遇的纤维关节，仅在头骨中发现。它们可以使颅面骨纵向生长，没有它们，头骨可以变厚但不能变长。硬脑膜在颅骨缝线和颅骨形成的形态发生和表型维持中起着重要作用。大脑，硬脑膜，缝线间充质和颅骨之间组织相互作用的任何改变（遗传或环境）都会影响颅骨的形成和/或缝线过早闭塞（颅突）。硬脑膜和颅缝线在脑扩张，颅骨骨形成和骨愈合中作用的复杂性导致人们假设在这些组织中存在间充质干细胞。分离新生儿硬脑膜和颅缝线细胞，将其培养并分化为几个限制性谱系。这些细胞在特定刺激下能够经历成骨，成软骨和成脂分化。成年硬脑膜细胞不能分化为软骨细胞和脂肪细胞。 Stro-1抗体的阳性染色和血流定量分析进一步支持了新生儿硬脑膜和颅骨缝线组织内具有多种分化潜能的细胞的存在。然而，成年硬脑膜不能表达Stro-I抗原。总之，新生硬脑膜和颅骨缝线组织中存在具有间充质干细胞特征的细胞亚群，并且该群体显着减少或在成年硬脑膜组织中不存在。

著录项

  • 作者

    Peptan, Ioana Alexandra.;

  • 作者单位

    University of Illinois at Chicago.;

  • 授予单位 University of Illinois at Chicago.;
  • 学科 Engineering Biomedical.; Health Sciences Medicine and Surgery.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 99 p.
  • 总页数 99
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物医学工程;
  • 关键词

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